| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1244-2 | ||||
Resumo:The discovery of new microbial strains with biotechnological potential is related to the ability of microorganisms to adapt to a variety of environments. The greater the chemical complexity of this environment, the greater the existence of microbial samples producing biomolecules of biotechnological interest. Thus, the enormous applicability and variability of lipolytic enzymes and biosurfactants among producing microorganisms justifies the search for new strains. In this context, this study aimed to select filamentous fungi that produce lipolytic enzymes and biosurfactants, isolated from an area impacted by oil spill and from another area without contamination, both in the mangrove sediments of Rio Formoso, Pernambuco. Thirty-five fungal samples were investigated, representatives of the genera Aspergillus, Penicillium, Trichoderma and Talaromyces, which are kept in the UCP Culture Collection, of the Nucleus of Research in Environmental Sciences and Biotechnology (NPCIAMB) of the Catholic University of Pernambuco, registered by number 927, in the World Federation for Culture Collections (WFCC). The strains were inoculated in Petri dishes containing phenol red agar with 1% Tween 80 and plates containing phenol red agar with 1% Tween 20, for detection of lipase and esterase, respectively. The principle of the method is the change in color from red to yellow with the decrease in pH resulting from the release of fatty acids after lipolysis. Biosurfactant production was analyzed on blood agar plates, observing a hemolysis halo around the colony. According to the results, seven fungi showed lipolytic activity, with emphasis on Palavras-chave: Bioremediation, lipase, esterase, surfactants Agência de fomento:FACEPE (project APQ-0638-2.01/19), CNPq and CAPES. | |||||